Professor Bruce Roe

Office: SRTC 2107
Phone: 405-325-4912
Email: broe@ou.edu
Group Homepage Link
Full Publications List and Vitae

Bruce A. Roe

George Lynn Cross Research Professor.
B.A. (Hope College) 1963
M.A., Ph.D. (Western Michigan) 1967, 1970.
NIH Postdoctoral Fellow (SUNY at Stony Brook) 1970-1973
NIH Research Career Development Award, 1976-1981
Regents' Award for Superior Accomplishment in Research, 1984
George Lynn Cross Research Professor, 1997

Division:

Biochemistry

Research Interests

Nucleic acid structure and function;cloning, sequence, and expression of mammalian mitochondrial and cytoplasmic tRNAs; mammalian protein synthesis; alterations in tRNA structure during normal and abnormal cellular development; modified nucleotides in mammalian tRNAS.

Research Description

Our laboratory presently is one of the NIH designated Centers involved in the Human Genome Project, whose goal is to determine the complete sequence of the 3 Billion base paired human chromosomes by 2005. During the past decade we have played a major role in developing many of the techniques needed to complete this goal, as well as trained many of the MS and PhD scientists actively involved in this project worldwide, and implementing new robotics and computer based analysis methods. We also are sequencing several bacterial genomes and actively discovering the new and unique genes in both humans and bacteria. These studies have and will continue to provide an in-depth genetic based understanding of normal gene function and the alterations that occur in a myriad of genetic-based diseases.

Come visit us at the Chromosome 22 site where you will find lots of detail about our work and the people in the lab.

Selected Recent Publications

R. H. Waterston, K. Lindblad-Toh, E. Birney, J. Rogers, et al. Initial sequencing and comparative analysis of the mouse genome. Nature 420, 520 - 562 (2002).

D. Ajdic, W. M. McShan, R. E. McLaughlin, G. Savic, J. Chang, M. B. Carson, C. Primeaux, R. Tian, S. Kenton, H. Jia, S. Lin, Y. Qian, S., Li, H. Zhu, F. Najar, H. Lai, J. White, Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. B. A. Roe, and J. J., Ferretti. Proceeding National Academy Science 99, 14434-14439 (2002).

J. Kone, J. Arroyo, T. Savinelli, S. Lin, K. Boyd, Y. Wu, M. Nimmakayalu, N.G. Copeland, N.A. Jenkins, M. Qumsiyeh, P. Hu, A. Prescott,, H. Wu, L. Yang L, B.A. Roe and A.S. Perkins. F-MuLV acceleration of myelomonocytic tumorigenesis in SV40 large T antigen transgenic mice is accompanied by retroviral insertion at Fli1 and a novel locus, Fim4. Leukemia 16(9):1827-34 (2002)

K. Sossey-Alaoui, G. Su, E. Malaj, B.A. Roe and J.K. Cowell. WAVE3, an actin-polymerization gene, is truncated and inactivated as a result of a constitutional t(1;13)(q21;q12) chromosome translocation in a patient with ganglioneuroblastoma. Oncogene 21(38):5967-74 (2002)

E.S. Srivatsan, R. Chakrabarti, K. Zainabadi, S.D. Pack, P. Benyamini, M.S. Mendonca, P.K. Yang, K. Kang, D. Motamedi, M.P. Sawicki, Z. Zhuang, R.A. Jesudasan, U. Bengtsson, C. Sun, B.A. Roe, E.J. Stanbridge, S.P. Wilczynski and J.L. Redpath. Localization of deletion to a 300 Kb interval of chromosome 11q13 in cervical cancer. Oncogene 21(36):5631-42 (2002)

K. Rithidech, J.J. Dunn, B.A. Roe, C.R. Gordon and E.P. Cronkite. Evidence for two commonly deleted regions on mouse chromosome 2 in gamma ray-induced acute myeloid leukemic cells. Exp Hematol 30(6):564-70 (2002)

H. Wang, B. Ding, C.J. Liu, X.Y. Ma, S. Deschamps, B.A. Roe and P. Lengyel. The Increase in Levels of Interferon-Inducible Proteins p202a and p202b and RNA-Dependent Protein Kinase (PKR) During Myoblast Differentiation Is Due to Transactivation by MyoD: Their Tissue Distribution in Uninfected Mice Does Not Depend on Interferons. J Interferon Cytokine Res 22(6):729-37 (2002)

J. J. Iandolo, V. Worrell, K.H. Groicher, Y. Qian, R. Y. Tian, S. Kenton, A. Dorman, H-G. Jia, S. Lin, P. Loh, S. Qi, H. Zhu and B.A. Roe. Comparative analysis of the genomes of the temperate bacteriophages phi 11, phi 12 and phi 13 of Staphylococcus aureus 8325. Gene 2002 May 1;289(1-2):109-18

Q. Zhang, W. Li, E.K. Novak, A. Karim, V.S. Mishra, S.F. Kingsmore, B.A. Roe, T. Suzuki and R.T. Swank. The gene for the muted (mu) mouse, a model for Hermansky-Pudlak syndrome, defines a novel protein which regulates vesicle trafficking. Hum Mol Genet 2002 Mar 15;11(6):697-706

G. Szalai, G. Duester, R. Friedman, H. Jia, S. Lin, B.A. Roe and M.R. Felder. Organization of six functional mouse alcohol dehydrogenase genes on two overlapping bacterial artificial chromosomes. Eur J Biochem 2002 Jan;269(1):224-32

R.E. Broughton, J.E. Milam and B.A. Roe. Complete Nucleotide Sequence of the Zebrafish (Danio rerio) Mitochondrial Genome and Evolutionary Patterns in Vertyebrate Mitochondrial DNA. Genome Research 11, 1958-1967 (2001).

T. Suzuki, W. Li, Q. Zhang, E.K. Novak, E.V. Sviderskaya, A. Wilson, D. C. Bennett, B.A. Roe, R.T. Swank and R.A. Spritz. The gene mutated in cocoa mice, carrying a defect of organelle biogenesis, is a homologue of the human Hermansky-Pudlak syndrome-3 gene. Genomics 78(1-2):30-7 (2001).

I. Tapia-Paez, M. Kost-Alimova, P. Hu, B.A. Roe, E. Blennow, L. Fedorova, S. Imrek, and J.P. Dumanski. The position of t(11;22)(q23;q11) constitional translocation breakpoint is conserved among its carriers. Human Genetics. 109, 167-177 (2001).

O. B. Chernova, A. Hunyadi, E. Malaj, H. Pan, C. Crooks, B. Roe and J. K. Cowell. A novel member of the WD-repeat gene family, WDR11, maps to the 10q26 region and is disrupted by a chromosome translocation in human glioblastoma cells. Oncogene 20, 5378-5392 (2001).

T.K. Footz, P. Brinkman-Mills, G.S. Banting, S.A. Maier, M. A. Riazi, L. Bridgland, S. Hu,, B. Birren, S. Minoshima, N. Shimizu, H.Q., Pan, T. Nguyen, F. Fang, Y. Fu, L. Ray, H. Wu, S. Shaull, S. Phan, Z. Yao, F. Chen, A. Hua, P. Hu, Q. Wang, P. Loh, S. Qi, B.A. Roe and H.E. McDermid. Analysis of the cat eye syndrome critical region in humans and the region of conserved synteny in mice: A search for candidate genes at or near the human chromosome 22 pericentromere. Genome Research 11, 1053-1070 (2001).

M. Levine, A. Progulske-Fox, N.D. Denslow, W.G. Farmerie, D.M Smith, W.T. Swearingen, F.C. Miller, Z. Liang, B.A. Roe and H-Q. Pan., Identification of Lysine decarboxylase as a Mammalian Cell Growth Inhibitor in Eikenella corrodens: Possible Role in Periodontal Disease. Microbial Pathogens 30, 179-192 (2001).

W. Ji, F. Chen, T. Do, A. Do, B. A. Roe and M. H. Meisler. DQX1, an RNA dependent ATPase homolog with a novel DEAQ box: expression pattern and genomic sequence comparison of the human and mouse genes. Mammalian Genome 12, 456-461 (2001).

J.J. Ferretti, W.M. McShan, D. Ajdic, D. J. Savic, G. Savic, K. Lyon, S. Sezate, A. N. Suvorov, C. Primeaux, S. Kenton, H. Lai, S. Lin, Y., Qian, H. Jia, H. Zhu, Q. Ren, F.Z, Najar, L. Song, J. White, X. Yuan, S. W. Clifton, B. A. Roe, R. McLaughlin. Complete Genome Sequence of an M1 Strain of Streptococcus pyogenes Proc. Natl. Acad. Sci. USA. 98, 4658-4663 (2001).

H. Zhu, M. Nowrousian, D. Kupfer, H.V. Colot, G. Berrocal-Tito, H. Lai, D. Bell-Pedersen, B.A. Roe, J.J. Loros, J.C. Dunlap. Analysis of Expressed Sequence Tags From Two Starvation, Time-of-Day-Specific Libraries of Neurospora crassa Reveals Novel Clock-Controlled Genes. Genetics. 157, 1057-1065 (2001).

E.S. Lander, J. Rogers, R. H. Waterson, T. Hawkins, R. Gibbs, Y. Sakaki, J. Weissenbach, D. R. Smith, A. Rosenthal, H. Yang, L. Hood, R., W. Davis R. M. Myers, M. V. Olson, N. Shimizu, G. A. Evans, B. A. Roe, J. Ramser, W. R. McCombie, H. Blocker, R. Agarwala, F. Collins and other members of the International Human Genome Sequencing Consortium. Initial Sequencing and Analysis of the Human Genome. Nature 409, 860-921 (2001).

E. Dawson , Y. Chen, S. Hunt, L.J. Smink, A. Hunt, K. Rice, S. Livingston, S. Bumpstead, R. Bruskiewich, P, Sham, R. Ganske, M. Adams, K. Kawasaki, N. Shimizu, S. Minoshima, B. Roe, D. Bentley, I. Dunham. A SNP Resource For Human Chromosome 22: Extracting Dense Clusters of SNPs from the Genomic Sequence. Genome Research, 11, 170-178 (2001).

E. Kitamura, G. Su, K. Sossey-Alaoui, E. Malaj, J. Lewis, H. Pan, L. Hawthorn, B.A. Roe and J. K. Cowell A transcription map of the minimally deleted region from 13q14 in B-cell chronic lymphocytic leukemia as defined by large scale sequencing of the 620 kb critical region. Oncogene, 19, 5772-5780 (2000).

P.L. Strissel, R. Strick, R.J. Tomek, B.A. Roe, J.D. Rowley and N.J. Zeleznik-Le. DNA structural properties of AF9 are similar to MLL and could act as recombination hot spots resulting in MLL/AF9 translocations and leukemogenesis. Human Molecular Genetics, 9, 1671-1679 (2000).

M. Pletcher, B. A. Roe, F. Chen, T. Do, A. Do, E. Malaj and R. Reeves. Chromosome evolution: The junction of mammalian chromosomes in the formation of mouse chromosome 10. Genome Research. 10, 1463-1467 (2000).

A. Raas-Rothschild, V. Cormier-Daire, M. Bao, E. Genin, R. Salomon, K. Brewer, M. Zeigler, H. Mandel, S. Toth, B. Roe, A. Munnich, and W.M. Canfield. Molecular basis of variant pseudo-hurler polydystrophy. J. Clinical Investigation 105, 673-681 (2000).

M. Siniscalco, R. Robledo, S. Orru, L. Contu, P. Yadav, Q. Ren, H. Lai, and B. Roe. A plea to search for deletion polymorphism through genome scans in populations. Trends in Genetics 16, 435-467 (2000).

I. Tapia-Paez, K.P. O'Brien, M. Kost-Alimova, S. Sahlen S, D. Kedra, C.E. Bruder, B. Andersson, B.A. Roe, P. Hu, S. Imreh, E. Blennow, and J.P. Dumanski. Fine mapping of the constitutional translocation t(11;22). Hum. Genet. 106, 506-516 (2000).

C.J. Liu, H. Wang, Z. Zhao, S. Yu, Y. Lu, J. Meyer, G, Chatterjee, S. Deschamps, B.A. Roe, and P. Lengyel. MyoD-Dependent Induction during Myoblast Differentiation of p204, a Protein Also Inducible by Interferon. Mol. Cell. Biol. 20, 7024-7036 (2000).

H. Kurahashi, T.H. Shaikh, P. Hu, B.A. Roe, B.S. Emanuel, and M.L. Budarf. Regions of genomic instability on 22q11 and 11q23 as the etiology for the recurrent constitutional t(11;22) Human Molecular Genetics 9, 1665-1670,(2000).

R.J. Moreland, M.E. Dresser, J.S. Rodgers, B.A. Roe, J.W. Conaway, R.C. Conaway and J.S. Hanas. Identification of a transcription factor IIIA-interacting protein. Nucleic Acids Research 28, 1986-1993 (2000).

J. R. Korenberg, X-N. Chen, H. Hirota, Z. Lai, U. Bellugi, D. Burian, B. Roe and R. Matsuoka. Genome Structure and Cognitive Map of Williams Syndrome. J. Cognitive Neuroscience 12, 89-107, 2000. D. V. Santi, M. A. Siani, B. Julien, D. Kupfer and B. Roe. An approach genes: a search for the epothilone gene cluster. Gene 247, 97-102 (2000).

M.A. Riazi, P. Brinkman-Mills, T. Nguyen, H. Pan, S. Phan, F. Ying, B. A. Roe, J. Tochigi, Y. Shimizu, S. Minoshima, N. Shimizu, M. Buchwald, and H.E. McDermid. The human homologue of insect-derived growth factor, CECR1, is a candidate gene for features of cat eye syndrome. Genomics 64, 277-285 (2000).

M. Luijten, Y.P. Wang, B.T. Smith, A. Westerveld, L.J. Smink, I, Dunham, B.A. Roe and T.J. Hulsebos. Mechanism of spreading of the highly related neurofibromatosis type 1 (NF1) pseudogenes on chromosomes 2, 14 and 22. European Journal of Human Genetics 8, 209-214 (2000).

K.L. Hester, J. Lehman, F. Najar, L. Song, B.A. Roe, C.H. MacGregor, P.W. Hager, P.V. Phibbs and J.R. Sokatch. Crc Is Involved in Catalobite Repression Control of the bkd Operons of Pseudomonas putida and Pseudomonas aeruginosa. J. Bacteriology 182, 1144-1149 (2000).

J. Lund, F. Chen. A. Hua, B. Roe, M. Budarf, B.S. Emanuel and R.H. Reeves. Comparative Sequence Analysis of 634Kbp of the Mouse Chromosome 16 Region of Conserved Synteny with the Human Velocardiofacial Region of Chr 22q11.2. Genomics 63. 374-383 (2000).

T.H. Shaikh, H. Kurahashi, S.C. Saitta, A. M. O'Hare, P. Hu, B.A. Roe, D.A. Driscoll, D.M. McDonald-McGinn, E.H. Zackai, M.L. Budarf and B.S. Emanuel. Chromosome 22-specific low copy repeats and the 22q11.2 deletion syndrome: genomic organization and deletion endpoint analysis. Human Molecular Genetics, 9(4), 489-501 (2000).

G. Glusman, A. Sosinsky, E, Ben-Asher, N. Avidan, D. Sonkin, A. Bahar, A. Rosenthal, S. Clifton, B. Roe, C. Ferraz, J. Demaille, and D. Lancet. Sequence, Structure, and Evolution of a Complete Human Olfactory Receptor Gene Cluster. Genomics 63(2): 227-245 (2000).

J.Y. Zhou, B. Fogelgren, Z. Wang, B. A. Roe and J. A. Biegel. Isolation of genes from the rhabdoid tumor deletion region in chromosome band 2q11.2 Gene 241, 133-141 (2000).

I. Dunham, N. Shimizu, B.A. Roe, S. Chissoe, et. al. The DNA Sequence of Human Chromosome 22. Nature 402, 489-495 (1999).

University of Oklahoma Department of Chemistry and Biochemistry 620 Parrington Oval, Rm 208 Norman, OK 73019-3051